Protein_Domain

Part:BBa_K2730000:Design

Designed by: Xinyue Ma   Group: iGEM18_Tongji_China   (2018-10-08)


Type III secretion chaperone-ExoS54-Flag-MSC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 429


Design Notes

Following the flag, there is sequence of MSC(SacI-KpnI-XbalI-SalI-HindIII) for adding.


Source

The domains of chaperone, promotor region and ExoS54 comes from natural Pseudomonas aeruginosa.

References